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Genetic variation study in 11 populations of D. chrysotoxum was conducted using 20 different inter-simple sequence repeats (ISSR) and 9 start codon targeted (SCoT) primers. Out of 199 bands produced by ISSR markers, 196 bands were polymorphic, resulting in high polymorphism (98.49%). SCoT markers, however, produced 111 polymorphic fragments generating 100% polymorphism. The Nei's genetic diversity at the species level (H = 0.184±0.11 for ISSR; H = 0.236±0.12 for SCoT) was higher than the average value at the population level (H = 0.160±0.15 for ISSR; H = 0.174±0.15 for SCoT). Low genetic differentiation among the populations (Gst = 0.1566 for ISSR; Gst = 0.2677 for SCoT) was observed due to high estimate of gene flow (Nm = 2.6938 for ISSR; Nm = 1.3678 for SCoT). Mantel test revealed a non-significant correlation between the genetic and geographical distance (r = -0.62; p = 0.86 for ISSR; r …