作者
Ana Caterina Parente-Pereira, Jerome Burnet, David Ellison, Julie Foster, David Marc Davies, Sjoukje Van der Stegen, Sophie Burbridge, Laura Chiapero-Stanke, Scott Wilkie, Stephen Mather, John Maher
发表日期
2011/8
期刊
Journal of clinical immunology
卷号
31
页码范围
710-718
出版商
Springer US
简介
Adoptive immunotherapy using chimeric antigen receptor-engrafted T cells is a promising emerging therapy for cancer. Prior to clinical testing, it is mandatory to evaluate human therapeutic cell products in meaningful in vivo pre-clinical models. Here, we describe the use of fused single-photon emission CT–CT imaging to monitor real-time migration of chimeric antigen receptor-engineered T cells in immune compromised (SCID Beige) mice. Following intravenous administration, human T cells migrate in a highly similar manner to that reported in man, but penetrate poorly into established tumors. By contrast, when delivered via intraperitoneal or subcutaneous routes, T cells remain at the site of inoculation with minimal systemic absorption—irrespective of the presence or absence of tumor. Together, these data support the validity of pre-clinical testing of human T-cell immunotherapy in SCID Beige mice. In …
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AC Parente-Pereira, J Burnet, D Ellison, J Foster… - Journal of clinical immunology, 2011