作者
Pei Yun Teo, Chuan Yang, Lynsey M Whilding, Ana C Parente‐Pereira, John Maher, Andrew JT George, James L Hedrick, Yi Yan Yang, Sadaf Ghaem‐Maghami
发表日期
2015/6
期刊
Advanced healthcare materials
卷号
4
期号
8
页码范围
1180-1189
简介
Adoptive T cell immunotherapy is a promising treatment strategy for epithelial ovarian cancer (EOC). However, programmed death ligand‐1 (PD‐L1), highly expressed on EOC cells, interacts with programmed death‐1 (PD‐1), expressed on T cells, causing immunosuppression. This study aims to block PD‐1/PD‐L1 interactions by delivering PD‐L1 siRNA, using various folic acid (FA)–functionalized polyethylenimine (PEI) polymers, to SKOV‐3‐Luc EOC cells, and investigate the sensitization of the EOC cells to T cell killing. To enhance siRNA uptake into EOC cells, which over express folate receptors, PEI is modified with FA or PEG–FA so that siRNA is complexed into nanoparticles with folate molecules on the surface. PEI modification with a single functional group lowers the polymer cytotoxicity compared to unmodified PEI. FA‐conjugated polymers increase siRNA uptake into SKOV‐3‐luc cells and decrease …
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PY Teo, C Yang, LM Whilding, AC Parente‐Pereira… - Advanced healthcare materials, 2015