作者
John Maher, Renier J Brentjens, Gertrude Gunset, Isabelle Rivière, Michel Sadelain
发表日期
2002/1/1
期刊
Nature biotechnology
卷号
20
期号
1
页码范围
70-75
出版商
Nature Publishing Group US
简介
Artificial receptors provide a promising approach to target T lymphocytes to tumor antigens. However, the receptors described thus far produce either an activation or a co-stimulatory signal alone, thus limiting the spectrum of functions accomplished by the genetically modified cells. Here we show that human primary T lymphocytes expressing fusion receptors directed to prostate-specific membrane antigen (PSMA) and containing combined T-cell receptor-ζ (TCRζ), and CD28 signaling elements, effectively lyse tumor cells expressing PSMA. When stimulated by cell-surface PSMA, retrovirally transduced lymphocytes undergo robust proliferation, expanding by more than 2 logs in three weeks, and produce large amounts of interleukin-2 (IL-2). Importantly, the amplified cell populations retain their antigen-specific cytolytic activity. These data demonstrate that fusion receptors containing both TCR and CD28 signaling …
引用总数
学术搜索中的文章
J Maher, RJ Brentjens, G Gunset, I Rivière, M Sadelain - Nature biotechnology, 2002