作者
Benildo S Cavada, Cláudia F Santos, Thalles B Grangeiro, Edson P Nunes, Patricia VP Sales, Ronaldo L Ramos, Flávia AM De Sousa, Clebia V Crisostomo, Juan J Calvete
发表日期
1998/10/1
期刊
Phytochemistry
卷号
49
期号
3
页码范围
675-680
出版商
Pergamon
简介
The protein, a galactose binding lectin made up of a misture of full length chains and endogenous C- and N-terminal fragments, was purified from Vatairea macrocarpa seeds and its properties were studied. A lectin from Vatairea macrocarpa Duke seeds (VML) was isolated using affinity chromatography on a guar gum column. The lectin, a glycoprotein without erythrocyte specificity, displays specificity to galactose and some derivatives. On SDS-polyacrylamide gels, V. macrocarpa seed lectin is composed of two major high-Mr bands of 34 and 32 kDa and two minor low-Mr bands of 22 and 13 kDa. N-Terminal sequencing showed that the 34, 32, and 13 kDa products possess identical N-terminal sequence, which display best similarity with the N-terminal portion of Robinia pseudoacacia lectins (RPL). On the other hand, the N-terminal sequence of the 22 kDa band can be aligned with an internal sequence of RPL …
引用总数
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BS Cavada, CF Santos, TB Grangeiro, EP Nunes… - Phytochemistry, 1998