作者
Piyathida Pongsiri, Kesmanee Praianantathavorn, Apiradee Theamboonlers, Sunchai Payungporn, Yong Poovorawan
发表日期
2012/5/1
期刊
Asian Pacific journal of tropical medicine
卷号
5
期号
5
页码范围
342-346
出版商
No longer published by Elsevier
简介
OBJECTIVE
To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.
METHODS
We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.
RESULTS
The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.
CONCLUSIONS
This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
学术搜索中的文章
P Pongsiri, K Praianantathavorn, A Theamboonlers… - Asian Pacific journal of tropical medicine, 2012