作者
Ivana Lojkić, Nina Krešić, Ivana Šimić, Tomislav Bedeković
发表日期
2015/12
期刊
BMC veterinary research
卷号
11
页码范围
1-6
出版商
BioMed Central
简介
Background
Bovine coronavirus (BCoV) together with bovine torovirus (BToV), both members of the Coronaviridae family, order Nidovirales are the most common viral enteric pathogens. Although studied separately, their joint occurrence and the molecular diversity in cattle in Croatia have not been investigated.
Methods
A survey is carried out on 101 fecal samples from diarrheic young and adult cattle during the 3-year period from i) one large dairy herd, ii) four small herds and iii) three nasal and paired fecal samples from calves with symptoms of respiratory disease. Samples were submitted to RT-PCR and sequencing for BCoV Nucleocapsid gene, BCoV Spike gene and BToV Spike gene.
Results
BCoV was detected in 78.8 % of fecal samples from symptomatic cattle and three nasal and paired fecal samples from …
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