作者
Soon Hee Kim, Gwangli Park, Hoon Hyun, Jeong Heon Lee, Yoshitomo Ashitate, Jungmun Choi, Gloria H Hong, Eric A Owens, Maged Henary, Hak Soo Choi
发表日期
2013/1/25
期刊
Biomedical materials
卷号
8
期号
1
页码范围
014110
出版商
IOP Publishing
简介
Longitudinal monitoring of cell migration, division and differentiation is of paramount importance in cell-based medical treatment. However, currently available optical techniques for tracing cell growth and tissue development are limited in applications due to genetic modification, toxicity and inaccurate detection when utilizing the visible spectrum. We have developed lipophilic near-infrared (NIR) fluorophores with high optical properties and a low background signal that allows longitudinal monitoring of cell proliferation and differentiation. Intracellular labeling efficacy was highly dependent on the physicochemical properties of fluorophores such as lipophilicity, charge, polar surface area and rotational bonds. Among the series of NIR cyanine fluorophores, ESNF 13 showed high solubility in aqueous buffer, high membrane penetration, low cytotoxicity and a long-term signal maintainability with a high signal intensity …
学术搜索中的文章
SH Kim, G Park, H Hyun, JH Lee, Y Ashitate, J Choi… - Biomedical materials, 2013