作者
Tengteng Tang, William Landis, Emeline Raguin, Peter Werner, Luca Bertinetti, Mason Dean, Wolfgang Wagermaier, Peter Fratzl
发表日期
2022/8
期刊
Advanced NanoBiomed Research
卷号
2
期号
8
页码范围
2100162
简介
During crucial growth stages of vertebrate long bones, calcified cartilage beneath the growth plate is anchored to bone by a third mineralized component, the cement line. Proper skeletal development is contingent on the interplay of these three constituents, yet their mineralization processes and structural interactions are incompletely understood, in part from limited knowledge of their meso‐ and nanoscale features. Herein, focused ion beam‐scanning electron microscopy (FIB‐SEM) with serial surface imaging is applied to examine the cartilage–bone interface of mouse femoral heads at an unprecedented scale: FIB‐SEM provides 3D, nanometer resolution of structural details for volumes encompassing metaphyseal calcified cartilage, bone, and the intervening cement line. A novel and complex structural network is revealed, comprising densely packed nanochannels smaller than bone canaliculi (≈10–50 nm …
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T Tang, W Landis, E Raguin, P Werner, L Bertinetti… - Advanced NanoBiomed Research, 2022