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Since aggregation of protein result into number of human diseases including diabetes mellitus, Huntington's and Alzheimer's disease, etc. Hence prevention of aggregation of a polypeptide is of great clinical importance. Human serum albumin (HSA) being major transporter serum protein was studied here in order to prevent its aggregation under extreme conditions. Sulfamethoxazole (SMZ) which is an antibiotic, caused significant inhibition of aggregation which was evident by number of biophysical techniques. Molecular docking was performed to elucidate the protein ligand binding site. In the presence of SMZ decrease in ThT, ANS and RLS fluorescence intensity suggested the inhibitory potency of this antibiotic. Further resistance to increment in the absorbance of Congo red and turbidity was observed even at elevated temperature. Circular dichroism also corroborated these results in retaining its secondary …