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To study bone marrow (BM) stromal damage in a mouse model of infusion-induced BM failure.

Sublethally irradiated CByB6F1 mice were infused with 5 × 10⁶ C57BL/6 (B6) lymph node (LN) cells. Recipient BM cells were taken at 3, 7, 10, and 14 days following LN infusion and were cultured in vitro in α-modified Eagle media for 2–3 weeks. Peripheral blood and was analyzed by complete blood counts while BM lymphocyte infiltration/expansion was analyzed by flow cytometry. Marrow cells from affected and control mice were mixed and cultured in vitro to test nonspecific stromal damage.

Donor lymphocytes infiltrated host BM within 3–7 days and expanded significantly between 7 and 10 days, concurrent with the development of leukopenia, thrombocytopenia, and marrow hypoplasia. BM cells from mice at 7, 10, and 14 days after B6-LN cell infusion were …