作者
Eleonora Foglio, Giovanni Puddighinu, Pasquale Fasanaro, Daniela D'Arcangelo, Giulietta A Perrone, David Mocini, Ciro Campanella, Luigi Coppola, Mariantonia Logozzi, Tommaso Azzarito, Francesca Marzoli, Stefano Fais, Luisa Pieroni, Valeria Marzano, Antonia Germani, Maurizio C Capogrossi, Matteo A Russo, Federica Limana
发表日期
2015/10/15
期刊
International journal of cardiology
卷号
197
页码范围
333-347
出版商
Elsevier
简介
Background
We recently demonstrated that epicardial progenitor cells participate in the regenerative response to myocardial infarction (MI) and factors released in the pericardial fluid (PF) may play a key role in this process. Exosomes are secreted nanovesicles of endocytic origin, identified in most body fluids, which may contain molecules able to modulate a variety of cell functions. Here, we investigated whether exosomes are present in the PF and their potential role in cardiac repair.
Methods and results
Early gene expression studies in 3 day-infarcted mouse hearts showed that PF induces epithelial-to-mesenchymal transition (EMT) in epicardial cells. Exosomes were identified in PFs from non-infarcted patients (PFC) and patients with acute MI (PFMI). A shotgun proteomics analysis identified clusterin in exosomes isolated from PFMI but not from PFC. Notably, clusterin has a protective effect on cardiomyocytes …
学术搜索中的文章
E Foglio, G Puddighinu, P Fasanaro, D D'Arcangelo… - International journal of cardiology, 2015