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White shrimp (Litopenaeus vannamei) waste is a good source of chitin. However, the chemical chitin extraction generates a large amount of environmental-pollution and increases partial deacetylation of chitin. Therefore, the use of biological extraction is an interesting alternative. This study aimed to isolate chitin by Trypsin and Ficin with/without sodium-metabisulfite and investigate their properties as compared to commercial chitin. Shell demineralization and deproteinization were done by lactic-acid and proteolytic-enzyme (0.1–0.05%w/w). The enzymatic deproteinized shells were subjected to mild-alkali treatment (0–2% NaOH; 60 °C and 30 min). After demineralization the ash content was decreased to ~1.1%. The combination of enzymatic and mild chemical treatments exhibited the 92% deproteinization. The enzymatic deproteinization was higher in presence of sodium-metabisulphite (p > 0.05). The CI of …