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Agrobacterium species are responsible for the production of the polysaccharide known as curdlan. The curdlan was produced from 10 isolates that were collected from a variety of local sources, including as agricultural soils, root nodules, and plant roots. The isolates were identified by examinations using morphological, microscopic, and biochemical tests. After testing the isolates to see which ones could produce the most curdlan, the A2 isolate finally emerged with a production capacity of 29.2 gL-1. According to the findings, the production of curdlan was increased using the modified medium that contained date juice at a concentration of 26.4 mL. 100 mL-1 of the production medium. The resultant yield was 30.7 gL-1, which was the highest possible yield. The identification of curdlan was validated by the utilization of FTIR, NMR, and HPLC techniques. The modified medium had the capability of being utilized in the production of curdlan from agricultural waste products.