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The wheat flour was substituted by 1%, 5% and 10% of banana peels flour (BPF) and studied the chemical composition, color of flour, rheological characteristics of flour samples and determination of viable cells of yeast through the dough period. The percentage of the protein and gluten was decreased when the substitution percentage of BPF was increased while carbohydrate percentage was increased. Additionally, the color of flour samples was (6.1, 7.0 and 7.5) Kent-Jones for Iraqi wheat flour was substituted by 1%, 5% and 10% of BPF. Most of the rheological characteristics of the flour samples improved after the substitution process, the 10% of substitution percentage was significantly higher (p ≤ 0.05) compared with the other substitution flour samples and the control (normal flour). The viability cells of Saccharomyces cerevisiae were increased after substituted by 1%, 5% and 10% of BPF through dough …