作者
Justyna Mikuła-Pietrasik, Angelika Kuczmarska, Błażej Rubiś, Violetta Filas, Marek Murias, Paweł Zieliński, Katarzyna Piwocka, Krzysztof Książek
发表日期
2012/6/1
期刊
Free Radical Biology and Medicine
卷号
52
期号
11-12
页码范围
2234-2245
出版商
Pergamon
简介
Resveratrol (3,4′,5-trihydroxy-trans-stilbene; RVT) is a natural phytoestrogen known to modulate the rate of senescence in cultured cells. The mechanism by which RVT affects this process is still elusive. In this paper we used human peritoneal mesothelial cells (HPMCs) to examine the effect of RVT (0.5 and 10μM) on their growth and senescence, with particular emphasis paid to parameters associated with oxidative stress. The results showed that RVT used at a concentration of 0.5μM (but not at 10μM) markedly improved HPMC growth capacity, as evidenced by elevated expression of PCNA antigen, augmented fraction of cells in the S phase of the cell cycle, and increased number of divisions achieved before senescence. These effects coincided with diminished expression and activity of senescence-associated β-galactosidase but were not associated with changes in the telomere length and an incidence of …
学术搜索中的文章
J Mikuła-Pietrasik, A Kuczmarska, B Rubiś, V Filas… - Free Radical Biology and Medicine, 2012