作者
Mukund G Adsul, Pooja Dixit, Jitendra K Saini, Ravi P Gupta, Sankara Sri Venkata Ramakumar, Anshu S Mathur
发表日期
2022/8
期刊
Biotechnology and Bioengineering
卷号
119
期号
8
页码范围
2167-2181
简介
Metabolite production by filamentous fungi hampered because of high viscosity generated during growth. Low viscosity fermentation by mold is one of the preferred ways of large scale enzymes production. Cellulolytic enzymes play a key role during the process of lignocellulosic biomass conversion. In this study, a mutant RC‐23‐1 was isolated through mutagenesis (diethyl sulfate followed by UV) of Trichoderma reesei RUT‐C30. RCRC‐23‐1 not only gave higher cellulase production but also generated lower viscosity during enzyme production. Viscosity of mutant growth was more than three times lower than parent strain. RC‐23‐1 shows unique, yeast‐like colony morphology on solid media and small pellet‐like growth in liquid media. This mutant did not spread like mold on solid media. This mutant produces cellulases constitutively when grown in sugars. Using only glucose, the cellulase production was 4.1 …
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