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The Kunlun Pass Basin, at the foothill of Yuzhu Mountain (6224 m asl and the highest peak of the Kunlun Range), records Plio-Pleistocene fine-grained sediments sandwiched between glacial moraines. We document a new vertebrate fossil assemblage, the Yuzhu Fauna, with 16 mammal and 2 fish species that provide insights into basin chronology as well as the paleoenvironment adjacent to alpine glaciations in the Pliocene, in which the mammals and fishes lived. The Yuzhu Fauna consists of the following eight small mammals: Petenyia sp., Aepyosciurus sp., Nannocricetus mongolicus, cf. Orientalomys sinensis, Mimomys n. sp., Prosiphneus cf. P. eriksoni, Ochotona minor, Ochotona cf. O. lagreli, and eight large mammals: Rhinocerotidae indet., Hipparion (Proboscidipparion) pater, Qurliqnoria sp., Bovidae indet., cf. Panthera blytheae, Hyaeninae indet., Vulpes qiuzhudingi, and aff. Arctomeles sp. Two cyprinid …