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In this study, we examined gender and age differences in physical performance in football. Thirty-four elite female and 34 elite male players (age 17 ± 1.6 to 24 ± 3.4 years) from a professional football club were divided into four groups (n = 17 each) according to gender and competitive level (senior males, senior females, junior males, and junior females). Players were tested for specific endurance (Yo-YoIR1), sprint over 15 m (Sprint-15m), vertical jump without (CMJ) or with (ACMJ) arm swing, agility (Agility-15m), and ball dribbling over 15 m (Ball-15m). The Yo-YoIR1 and Agility-15m performances showed both a gender and competitive level difference (P < 0.001). Senior and junior males covered 97 and 153% more distance during the Yo-YoIR1 than senior and junior females, respectively (P < 0.001). Gender but not age differences were found for Sprint-15m performance (P < 0.001). No difference in …