作者
Pu Guo, Shengyong Yan, Jianlin Hu, Xiwen Xing, Changcheng Wang, Xiaowei Xu, Xiaoyu Qiu, Wen Ma, Chunjiang Lu, Xiaocheng Weng, Xiang Zhou
发表日期
2013/7/5
期刊
Organic letters
卷号
15
期号
13
页码范围
3266-3269
出版商
American Chemical Society
简介
Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.
学术搜索中的文章
P Guo, S Yan, J Hu, X Xing, C Wang, X Xu, X Qiu… - Organic letters, 2013