作者
Wenting Liu, Han Lai, Rong Huang, Chuntao Zhao, Yimo Wang, Xiaocheng Weng, Xiang Zhou
发表日期
2015/6/15
期刊
Biosensors and Bioelectronics
卷号
68
页码范围
736-740
出版商
Elsevier
简介
DNA methylation has received a large amount of attention due to its close relationship to a wide range of biological phenomena, such as gene activation, gene imprinting, and chromatin stability. Herein, we have designed a hairpin-shaped DNA probe with 5’-C-rich/G-rich-3’ tails and developed a simple and reliable fluorescence turn-off assay for DNA adenine methylation (Dam) methyltransferase (MTase) detection combining site recognition and the fluorescence enhancement of DNA-templated silver nanoclusters (DNA–AgNCs) by guanine-rich DNA sequences. A designed hairpin probe with 5’ CCCTTACCCC and 3’ GGGTGGGGTGGGGTGGGG displays a bright red emission after reacting with AgNO3 and NaBH4. In the presence of Dam MTase, the methylation-sensitive restriction endonuclease Dpn I which has the same recognition site with the Dam MTase can split the probe, freeing the G-rich sequence from …
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W Liu, H Lai, R Huang, C Zhao, Y Wang, X Weng… - Biosensors and Bioelectronics, 2015