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Effects of the exogenously added polyamines putrescine, spermidine and spermine and the inhibitors of polyamine biosynthesis, methylglyoxal bis(guanylhydrazone) (MGBG) and dicyclohexylamine (DCHA), in the DCR medium on the endogenous polyamine levels and growth of embryogenic callus of Scots pine (Pinus sylvestris L.) were studied. With polyamine treatments the endogenous putrescine level was signifi-candy affected only by 10 umol/L spermidine, which decreased the putrescine. Other polyamine treatments did not have an effect on the putrescine level. Endogenous spermidine or spermine levels were not significantly affected by exogenous polyamines, although lower spermine concentrations were found in callus tissues with all polyamine supplements. DCHA significantly reduced the endogenous putrescine level but not the spermidine or spermine levels. The highest concentration of MGBG (5 …