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Cold atmospheric plasma (CAP) possesses the ability of high-efficiency disinfection. It is reported that mixtures of reactive oxygen species (ROS) including ^·OH, ¹O₂, O₂⁻ and H₂O₂ generated from CAP have better antimicrobial ability than mimicked solution of mixture of single ROS type, but the reason is not clear. In this study, CAP was applied to treat yeasts in water in order to investigate the fungal inactivation efficiency and mechanism. The results showed that plasma treatment for 5 min could result in >2-log reduction of yeast cells, and application of varied ROS scavengers could significantly increase the yeast survival rate, indicating that ^·OH and ¹O₂ played the pivotal role in yeast inactivation. Moreover, the synergistic effect of ¹O₂ with other plasma-generated ROS was revealed. ¹O₂ could diffuse into cells and induce the depolarization of mitochondrial membrane potential (MMP), and different levels of MMP …