作者
Lu Cao, Yanming Zhou, Beibei Zhai, Jian Liao, Wen Xu, Ruixiu Zhang, Jing Li, Yu Zhang, Lei Chen, Haihua Qian, Mengchao Wu, Zhengfeng Yin
发表日期
2011/12
期刊
BMC gastroenterology
卷号
11
页码范围
1-11
出版商
BioMed Central
简介
Background
Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.
Methods
Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.
Results
The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D …
学术搜索中的文章
L Cao, Y Zhou, B Zhai, J Liao, W Xu, R Zhang, J Li… - BMC gastroenterology, 2011