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The pathogenesis of chronic hepatitis C virus (HCV) infection remains unclear. Tumour necrosis factor alpha (TNF‐α) is alleged to contribute in the pathogenesis of chronic HCV infection. Single nucleotide polymorphism in TNF‐α and ‐β genes could influence the outcome of HCV infection. The aim was to study single nucleotide polymorphism in TNF‐α promoter region and Nco I polymorphisms in the TNF‐β gene in patients with chronic hepatitis C. Fifty‐two patients with histologically proven chronic hepatitis, who had raised ALT levels (>1.5 × ULN) and were HCV RNA positive, were studied. Genotyping of −308 promoter variant of TNF‐α was performed by PCR with primers that incorporated an Nco I restriction site. For PCR typing of the TNF‐β Nco I restriction fragment length polymorphism, sequence specific primers were used. Polymorphism in the TNF‐α G/G, G/A and A/A allele was not different between …