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Computer simulations (CS) are applied for instrument design, feasibility tests and education in low-resource settings. These applications have been targeted by research biophotonics CS. However, software enabling these CS were not designed for education, as they require programming experience from users as well as specialized training to use it. In this study, we have developed and tested a diffuse-optics web application to teach tissue optics to undergraduate students with diverse learning styles and backgrounds. Students’ feedback showed that CS session using our web app received 84.6% “Very good” and ““Good” responses for overall learning, 76.9% for quality of the user manual instructions, 92.3% for quality of teaching, 100.0% for quality of subject matter among the participant respondents. Also, 92.3% of participants considered CS important for their learning process.