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Lupinus, known by the common name of lupine or lupin, is a highly diverse genus of legume and is one of the most geographically widespread plant genera. Lupines can develop indeterminate nodules, known as lupinoid-nodules, in association with rhizobia, mainly belonging to the Bradyrhizobium genus. In this work, diversity of Uruguayan native rhizobia obtained from nodules of native Lupinus species growing in their natural habitat, was evaluated. We generated a collection of 109 isolates obtained from nodules of six, out of the seven, Uruguayan native species of lupine described for Uruguay: Lupinus albescens, Lupinus bracteolaris, Lupinus gibertianus, Lupinus lanatus, Lupinus multiflorus and Lupinus paraguariensis. According to the phylogeny of the 16 S rRNA gene, isolates belong to six well defined clusters within the Bradyrhizobium genus. Four of these clades include Bradyrhizobium species already …