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Lentil (Lens culinaris) is an important legume crop worldwide, consumed as dried seeds. Correct identification of lentil varieties is important in order to ensure food quality, safety, authenticity and health for consumers as well as high price from elite varieties for farmers and industry. Recently, DNA-based methods like the molecular markers microsatellites (SSRs) for nuclear DNA or the DNA barcoding which uses chloroplast or nuclear DNA have been developed for plant species or variety identification, for genotyping and for identification of their ingredients in the final food products. Here we have integrated High Resolution Melting (HRM) analysis, coupled with five SSR markers in parallel with rpoC1 chloroplast DNA barcode targeting region, in order to facilitate the identification of Protected Geographical Indication (PGI) lentil variety ‘Eglouvi’. The five SSR loci used were informative and generated a unique melting …