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In Pakistan, there is no screening for active or latent Tuberculosis in the health care system. This study serves to measure the prevalence of Latent tuberculosis infection in District Sargodha, Pakistan and evaluate possible clinical implications and treatment strategies for latent tuberculosis. This study also finds out the risk factors of Latent tuberculosis infection in District Sargodha. A structured questionnaire, administered in the supervision of research committee, Department of Zoology, The University of Lahore, Sargodha Campus, included information on latent tuberculosis infection, social contact, BCG, skin reaction, drugs, HIV, immune response, kidney, diabetes, food, intestinal, night sweats, fatigue, weight loss, chest pain, fever, breath, drug addiction, appetite, aneroxia, tenderness, spinal problems, use of allopathic and homeopathic medication, rash, partsia, sneezing and isoniozed, Interviews were conducted in private counseling rooms and the questionnaire were filled face to face with no correctional officer present to assure privacy and reduce perceived coercion. Results show that 67% of interviewers agreed that as a result of tuberculosis they face symptoms of TBI. 70% of patients of tuberculosis had experienced different allergies and reactions. 66% respondents agreed that they think that BCG can be the cause of Tuberculosis. Keeping in view the symptoms of contact almost 67% were respondents agreed with it that they think in the present research 64% agreed with it while 34% people thought that drugs cannot be the reason of Tuberculosis. Another important reason of different diseases ‘HIV/AIDS’was also one of the parameter of …