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Increasing resistance to antimicrobial agents used in the treatment of burns and wounds infections with Acinetobacter baumannii strains has become an important concern. Isolation and identification of A. baumannii strains from patients with burns and wounds infections by conventional and molecular methods and detection of their antimicrobial susceptibility. Antibiotics resistance of 96 A. baumannii strains isolated from the samples collected between February 2015 and June 2015 were evaluated. Identification of A. baumannii isolates were determined by CHROMagar Acinetobacter API 20E and automated system VITEK 2 and molecular methods by detection 16S rRNA and blaOXA-51 genes. Antimicrobial susceptibility tests were performed by agar disk diffusion and broth microdilution methods. Among 476 samples included burns and wounds infections, 96 isolates of A. baumannii were obtained, 65 belonged to burns and 31 from wounds. CHROMagar Acinetobacter had 100% sensitivity and specificity for isolation of A. baumannii. The PCR results showed that 16S rRNA sequence and blaOXA-51 gene exists in all A. baumannii collected from positive samples, and this confirmed the accuracy of other identification tests. In the present study, 87.5% of the isolates had Multi-Drug Resistance (MDR). It has been found that A. baumannii strains had high resistance to most antibiotics used: Cefotxime (87.5%), Ciprofloxacin (80.2%), Amikacin (79.2%), Meropenem (75%) Imipenem (81.3%) and Pipracillin (81.3%) but low resistance to Tigecycline (11.5%) and Colistin (7.3%). The present results indicate that antimicrobial resistance of A. baumannii in …