查看文章

We have demonstrated recently that Birbeck granule-positive Langerhans cells (LC) can be derived from CD34+ peripheral blood progenitor cells in the presence of a seven-cyto-kine cocktail (CC7-7). Here, we show that the sequential use of early-acting hematopoietic growth factors, stem cell factor, interleukin (IU-3, and IL-6, followed on day 8 by differentiation in the two-factor combination IL-4 plus granulocyte-macrophage colony-stimulating factor (GM-CSF) (CC4GMI is more efficient and allows the ceils to be arrested in the LC stage for more than 1 week while continuous maturation occurs in CC7-7. Maturation of LC to interdigitating dendritic cells (DC) could specifically be induced within 60 hours by addition of tumor necrosis factor-α (20 ng/mL) or lipopoiy-saccharide (100 ng/mL). Using LC that had been enriched to greater than 90% CD1a⁺ cells by an immunoaffinity column, we were able to define clear-cut …