Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system R Marshall, CS Maxwell, SP Collins, T Jacobsen, ML Luo, MB Begemann, ... Molecular cell 69 (1), 146-157. e3, 2018 | 219 | 2018 |
Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs T Jacobsen, F Ttofali, C Liao, S Manchalu, BN Gray, CL Beisel Nucleic acids research 48 (10), 5624-5638, 2020 | 44 | 2020 |
A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs CS Maxwell, T Jacobsen, R Marshall, V Noireaux, CL Beisel Methods 143, 48-57, 2018 | 42 | 2018 |
A facilitated diffusion mechanism establishes the Drosophila Dorsal gradient SN Carrell, MD O'Connell, T Jacobsen, AE Pomeroy, SM Hayes, ... Development 144 (23), 4450-4461, 2017 | 31 | 2017 |
The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs T Jacobsen, C Liao, CL Beisel FEMS microbiology letters 366 (8), fnz085, 2019 | 15 | 2019 |