| Isolating and engineering human antibodies using yeast surface display G Chao, WL Lau, BJ Hackel, SL Sazinsky, SM Lippow, KD Wittrup Nature protocols 1 (2), 755-768, 2006 | 1112 | 2006 |
| Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling BJ Hackel, A Kapila, KD Wittrup Journal of molecular biology 381 (5), 1238-1252, 2008 | 274 | 2008 |
| Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular convergence with … D Lipovšek, SM Lippow, BJ Hackel, MW Gregson, P Cheng, A Kapila, ... Journal of molecular biology 368 (4), 1024-1041, 2007 | 176 | 2007 |
| Fmoc-based synthesis of peptide α-thioesters using an aryl hydrazine support JA Camarero, BJ Hackel, JJ De Yoreo, AR Mitchell The Journal of organic chemistry 69 (12), 4145-4151, 2004 | 167 | 2004 |
| Stability and CDR composition biases enrich binder functionality landscapes BJ Hackel, ME Ackerman, SW Howland, KD Wittrup Journal of molecular biology 401 (1), 84-96, 2010 | 139 | 2010 |
| Pharmacokinetically stabilized cystine knot peptides that bind alpha-v-beta-6 integrin with single-digit nanomolar affinities for detection of pancreatic cancer RH Kimura, R Teed, BJ Hackel, MA Pysz, CZ Chuang, A Sathirachinda, ... Clinical Cancer Research 18 (3), 839-849, 2012 | 129 | 2012 |
| Photoacoustic lifetime contrast between methylene blue monomers and self-quenched dimers as a model for dual-labeled activatable probes E Morgounova, Q Shao, BJ Hackel, DD Thomas, S Ashkenazi Journal of biomedical optics 18 (5), 056004-056004, 2013 | 118 | 2013 |
| Highly avid magnetic bead capture: an efficient selection method for de novo protein engineering utilizing yeast surface display M Ackerman, D Levary, G Tobon, B Hackel, KD Orcutt, KD Wittrup Biotechnology progress 25 (3), 774-783, 2009 | 116 | 2009 |
| Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties CM Pirie, BJ Hackel, MG Rosenblum, KD Wittrup Journal of Biological Chemistry 286 (6), 4165-4172, 2011 | 102 | 2011 |
| Production of Soluble and Active Transferrin Receptor-Targeting Single-Chain Antibody using Saccharomyces cerevisiae BJ Hackel, D Huang, JC Bubolz, XX Wang, EV Shusta Pharmaceutical research 23, 790-797, 2006 | 96 | 2006 |
| Alternative non-antibody protein scaffolds for molecular imaging of cancer LA Stern, BA Case, BJ Hackel Current opinion in chemical engineering 2 (4), 425-432, 2013 | 86 | 2013 |
| Use of 64Cu-labeled Fibronectin Domain with EGFR-Overexpressing Tumor Xenograft: Molecular Imaging BJ Hackel, RH Kimura, SS Gambhir Radiology 263 (1), 179-188, 2012 | 84 | 2012 |
| Multivalent ligand binding to cell membrane antigens: defining the interplay of affinity, valency, and expression density CM Csizmar, JR Petersburg, TJ Perry, L Rozumalski, BJ Hackel, ... Journal of the American Chemical Society 141 (1), 251-261, 2018 | 79 | 2018 |
| A 45-amino-acid scaffold mined from the PDB for high-affinity ligand engineering MA Kruziki, S Bhatnagar, DR Woldring, VT Duong, BJ Hackel Chemistry & biology 22 (7), 946-956, 2015 | 69 | 2015 |
| A novel engineered anti-CD20 tracer enables early time PET imaging in a humanized transgenic mouse model of B-cell non-Hodgkins lymphoma A Natarajan, BJ Hackel, SS Gambhir Clinical Cancer Research 19 (24), 6820-6829, 2013 | 68 | 2013 |
| 18F-Fluorobenzoate–labeled cystine knot peptides for PET imaging of integrin αvβ6 BJ Hackel, RH Kimura, Z Miao, H Liu, A Sathirachinda, Z Cheng, FT Chin, ... Journal of Nuclear Medicine 54 (7), 1101-1105, 2013 | 68 | 2013 |
| Engineering fibronectin-based binding proteins by yeast surface display TF Chen, S De Picciotto, BJ Hackel, KD Wittrup Methods in enzymology 523, 303-326, 2013 | 67 | 2013 |
| Engineered proteins including mutant fibronectin domains KD Wittrup, JB Spangler, BJ Hackel US Patent App. 13/390,086, 2012 | 59 | 2012 |
| The full amino acid repertoire is superior to serine/tyrosine for selection of high affinity immunoglobulin G binders from the fibronectin scaffold BJ Hackel, KD Wittrup Protein Engineering, Design & Selection 23 (4), 211-219, 2010 | 54 | 2010 |
| High-throughput ligand discovery reveals a sitewise gradient of diversity in broadly evolved hydrophilic fibronectin domains DR Woldring, PV Holec, H Zhou, BJ Hackel PLoS One 10 (9), e0138956, 2015 | 52 | 2015 |
