SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm J Lobstein, CA Emrich, C Jeans, M Faulkner, P Riggs, M Berkmen Microbial cell factories 11, 1-16, 2012 | 663 | 2012 |
Bacterial species exhibit diversity in their mechanisms and capacity for protein disulfide bond formation RJ Dutton, D Boyd, M Berkmen, J Beckwith Proceedings of the National Academy of Sciences 105 (33), 11933-11938, 2008 | 271 | 2008 |
Production of disulfide-bonded proteins in Escherichia coli M Berkmen Protein expression and purification 82 (1), 240-251, 2012 | 268 | 2012 |
The DsbA Signal Sequence Directs Efficient, Cotranslational Export of Passenger Proteins to the Escherichia coli Periplasm via the Signal Recognition Particle … CF Schierle, M Berkmen, D Huber, C Kumamoto, D Boyd, J Beckwith Journal of bacteriology 185 (19), 5706-5713, 2003 | 261 | 2003 |
The nonconsecutive disulfide bond of Escherichia coli phytase (AppA) renders it dependent on the protein-disulfide isomerase, DsbC M Berkmen, D Boyd, J Beckwith Journal of Biological Chemistry 280 (12), 11387-11394, 2005 | 165 | 2005 |
Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria MP Robinson, N Ke, J Lobstein, C Peterson, A Szkodny, TJ Mansell, ... Nature communications 6 (1), 8072, 2015 | 138 | 2015 |
Production of Disulfide‐Bonded Proteins in Escherichia coli N Ke, M Berkmen Current protocols in molecular biology 108 (1), 16.1 B. 1-16.1 B. 21, 2014 | 66 | 2014 |
Use of the SHuffle strains in production of proteins G Ren, N Ke, M Berkmen Current Protocols in Protein Science 85 (1), 5.26. 1-5.26. 21, 2016 | 61 | 2016 |
Visualization of periplasmic and cytoplasmic proteins with a self-labeling protein tag N Ke, D Landgraf, J Paulsson, M Berkmen Journal of bacteriology 198 (7), 1035-1043, 2016 | 58 | 2016 |
In vivo oxidative protein folding can be facilitated by oxidation–reduction cycling SR Shouldice, SH Cho, D Boyd, B Heras, M Eser, J Beckwith, P Riggs, ... Molecular microbiology 75 (1), 13-28, 2010 | 55 | 2010 |
Disulfide Bond Formation in the Periplasm of Escherichia coli B Manta, D Boyd, M Berkmen EcoSal Plus 8 (2), 10.1128/ecosalplus. ESP-0012-2018, 2019 | 48 | 2019 |
Visualizing bacterial colony morphologies using time-lapse imaging chamber MOCHA M Peñil Cobo, S Libro, N Marechal, D D'Entremont, D Peñil Cobo, ... Journal of bacteriology 200 (2), 10.1128/jb. 00413-17, 2018 | 24 | 2018 |
TrbB from conjugative plasmid F is a structurally distinct disulfide isomerase that requires DsbD for redox state maintenance CW Hemmis, M Berkmen, M Eser, JF Schildbach Journal of bacteriology 193 (18), 4588-4597, 2011 | 24 | 2011 |
Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents BP Anton, A Fomenkov, EA Raleigh, M Berkmen Genome Announcements 4 (2), 10.1128/genomea. 00230-16, 2016 | 22 | 2016 |
Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote WH Reuter, T Masuch, N Ke, M Lenon, M Radzinski, V Van Loi, G Ren, ... Redox biology 26, 101280, 2019 | 21 | 2019 |
RETRACTED ARTICLE: A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo D Mizrachi, MP Robinson, G Ren, N Ke, M Berkmen, MP DeLisa Nature chemical biology 13 (9), 1022-1028, 2017 | 21 | 2017 |
The Serratia marcescens NucE protein functions as a holin in Escherichia coli M Berkmen, MJ Benedik, U Bläsi Journal of bacteriology 179 (20), 6522-6524, 1997 | 18 | 1997 |
Disulfide-Bonded Protein Production in E. coli Involvement of Disulfide Bond Isomerase Improves Protein Folding JC Samuelson, TB Causey, M Berkmen Genetic Engineering & Biotechnology News 32 (3), 35-35, 2012 | 16 | 2012 |
Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli PT Reddy, RG Brinson, JT Hoopes, C McClung, N Ke, L Kashi, ... MAbs 10 (7), 992-1002, 2018 | 15 | 2018 |
RNase E, the major player in mRNA degradation, is down-regulated in Escherichia coli during a transient growth retardation (diauxic lag) T Barlow, M Berkmen, D Georgeliis, L Bayr, S Arvidson, A von Gabain Biological chemistry 379 (1), 33-38, 1998 | 15 | 1998 |