| Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review O Cos, R Ramón, JL Montesinos, F Valero Microbial cell factories 5, 1-20, 2006 | 464 | 2006 |
| Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris S Minning, A Serrano, P Ferrer, C Solá, RD Schmid, F Valero Journal of Biotechnology 86 (1), 59-70, 2001 | 237 | 2001 |
| Effects of methanol on lipases: molecular, kinetic and process issues in the production of biodiesel M Lotti, J Pleiss, F Valero, P Ferrer Biotechnology journal 10 (1), 22-30, 2015 | 172 | 2015 |
| The potential use of lipases in the production of fatty acid derivatives for the food and nutraceutical industries S Ferreira-Dias, G Sandoval, F Plou, F Valero Electronic Journal of Biotechnology 16 (3), 12-12, 2013 | 171 | 2013 |
| Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures O Cos, A Serrano, JL Montesinos, P Ferrer, JM Cregg, F Valero Journal of biotechnology 116 (4), 321-335, 2005 | 169 | 2005 |
| Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter D Resina, A Serrano, F Valero, P Ferrer Journal of biotechnology 109 (1-2), 103-113, 2004 | 136 | 2004 |
| Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris R Ramón, P Ferrer, F Valero Journal of Biotechnology 130 (1), 39-46, 2007 | 123 | 2007 |
| An improved secretion signal enhances the secretion of model proteins from Pichia pastoris JJ Barrero, JC Casler, F Valero, P Ferrer, BS Glick Microbial cell factories 17, 1-13, 2018 | 115 | 2018 |
| Physiological control on the expression and secretion of Candida rugosa lipase M Lotti, S Monticelli, JL Montesinos, S Brocca, F Valero, J Lafuente Chemistry and physics of lipids 93 (1-2), 143-148, 1998 | 107 | 1998 |
| Reactivity of Pure Candida rugosa Lipase Isoenzymes (Lip1, Lip2, and Lip3) in Aqueous and Organic Media. Influence of the Isoenzymatic Profile on the Lipase … N López, MA Pernas, LM Pastrana, A Sánchez, F Valero, ML Rúa Biotechnology Progress 20 (1), 65-73, 2004 | 97 | 2004 |
| Reaction scheme of lipase production byCandida rugosa growing on olive oil JL Del Rio, P Serra, F Valero, M Poch, C Sola Biotechnology Letters 12, 835-838, 1990 | 96 | 1990 |
| Biocatalytic synthesis of flavonoid esters by lipases and their biological benefits MEMB de Araújo, YEM Franco, MCF Messias, GB Longato, JA Pamphile, ... Planta medica 83 (01/02), 7-22, 2017 | 94 | 2017 |
| Developing high cell density fed‐batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source‐regulated FLD1 … D Resina, O Cos, P Ferrer, F Valero Biotechnology and Bioengineering 91 (6), 760-767, 2005 | 92 | 2005 |
| Effects of different fatty acids in lipase production by Candida rugosa N Obradors, JL Montesinos, F Valero, FJ Lafuente, C Sola Biotechnology letters 15, 357-360, 1993 | 91 | 1993 |
| Production of native and recombinant lipases by Candida rugosa A review P Ferrer, JL Montesinos, F Valero, C Solà Applied biochemistry and biotechnology 95, 221-255, 2001 | 89 | 2001 |
| Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures O Cos, D Resina, P Ferrer, JL Montesinos, F Valero Biochemical Engineering Journal 26 (2-3), 86-94, 2005 | 88 | 2005 |
| Highly enantioselective esterification of racemic ibuprofen in a packed bed reactor using immobilised Rhizomucor miehei lipase A Sánchez, F Valero, J Lafuente, C Solà Enzyme and microbial technology 27 (1-2), 157-166, 2000 | 88 | 2000 |
| Optimization of the heterologous production of a Rhizopus oryzae lipase in Pichia pastoris system using mixed substrates on controlled fed-batch bioprocess C Arnau, R Ramon, C Casas, F Valero Enzyme and microbial technology 46 (6), 494-500, 2010 | 83 | 2010 |
| Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction D Resina, M Bollók, NK Khatri, F Valero, P Neubauer, P Ferrer Microbial Cell Factories 6, 1-11, 2007 | 81 | 2007 |
| Recent patents on the Pichia pastoris expression system: expanding the toolbox for recombinant protein production M Bollok, D Resina, F Valero, P Ferrer Recent Patents on Biotechnology 3 (3), 192-201, 2009 | 80 | 2009 |
