| HaloTag: a novel protein labeling technology for cell imaging and protein analysis GV Los, LP Encell, MG McDougall, DD Hartzell, N Karassina, C Zimprich, ... ACS chemical biology 3 (6), 373-382, 2008 | 2429 | 2008 |
| Target engagement and drug residence time can be observed in living cells with BRET MB Robers, ML Dart, CC Woodroofe, CA Zimprich, TA Kirkland, ... Nature communications 6 (1), 10091, 2015 | 252 | 2015 |
| Quantitative, wide-spectrum kinase profiling in live cells for assessing the effect of cellular ATP on target engagement JD Vasta, CR Corona, J Wilkinson, CA Zimprich, JR Hartnett, MR Ingold, ... Cell chemical biology 25 (2), 206-214. e11, 2018 | 219 | 2018 |
| HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification RF Ohana, LP Encell, K Zhao, D Simpson, MR Slater, M Urh, KV Wood Protein expression and purification 68 (1), 110-120, 2009 | 170 | 2009 |
| Suppl 1: Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands LP Encell, RF Ohana, K Zimmerman, P Otto, G Vidugiris, MG Wood, ... Current chemical genomics 6, 55, 2012 | 142 | 2012 |
| HaloTag™ Interchangeable labeling technology for cell imaging and protein capture G Los, A Darzins, N Karassina, C Zimprich, R Learish, MG McDougall, ... Cell Notes 11, 2-6, 2005 | 95 | 2005 |
| HaloTag-based purification of functional human kinases from mammalian cells RF Ohana, R Hurst, J Vidugiriene, MR Slater, KV Wood, M Urh Protein expression and purification 76 (2), 154-164, 2011 | 78 | 2011 |
| Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor … LE Kilpatrick, R Friedman-Ohana, DC Alcobia, K Riching, CJ Peach, ... Biochemical Pharmacology 136, 62-75, 2017 | 57 | 2017 |
| Quantitative, real-time measurements of intracellular target engagement using energy transfer MB Robers, JD Vasta, CR Corona, RF Ohana, R Hurst, MA Jhala, ... Systems Chemical Biology: Methods and Protocols, 45-71, 2019 | 46 | 2019 |
| Deciphering the cellular targets of bioactive compounds using a chloroalkane capture tag R Friedman Ohana, TA Kirkland, CC Woodroofe, S Levin, HT Uyeda, ... ACS chemical biology 10 (10), 2316-2324, 2015 | 46 | 2015 |
| The luminescent HiBiT peptide enables selective quantitation of G protein–coupled receptor ligand engagement and internalization in living cells ME Boursier, S Levin, K Zimmerman, T Machleidt, R Hurst, BL Butler, ... Journal of Biological Chemistry 295 (15), 5124-5135, 2020 | 45 | 2020 |
| Quantifying target occupancy of small molecules within living cells MB Robers, R Friedman-Ohana, KVM Huber, L Kilpatrick, JD Vasta, ... Annual review of biochemistry 89 (1), 557-581, 2020 | 42 | 2020 |
| Real-time ligand binding of fluorescent VEGF-A isoforms that discriminate between VEGFR2 and NRP1 in living cells CJ Peach, LE Kilpatrick, R Friedman-Ohana, K Zimmerman, MB Robers, ... Cell chemical biology 25 (10), 1208-1218. e5, 2018 | 40 | 2018 |
| Complex Formation between VEGFR2 and the β2-Adrenoceptor LE Kilpatrick, DC Alcobia, CW White, CJ Peach, JR Glenn, K Zimmerman, ... Cell Chemical Biology 26 (6), 830-841. e9, 2019 | 37 | 2019 |
| Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression A Darzins, LP Encell, RF Ohana, P Otto, G Vidugiris, KV Wood, MG Wood, ... US Patent 8,420,367, 2013 | 35 | 2013 |
| Improved deconvolution of protein targets for bioactive compounds using a palladium cleavable chloroalkane capture tag R Friedman Ohana, S Levin, MG Wood, K Zimmerman, ML Dart, ... ACS chemical biology 11 (9), 2608-2617, 2016 | 34 | 2016 |
| Heteroduplex joint formation in Escherichia coli recombination is initiated by pairing of a 3′-ending strand R Friedman-Ohana, A Cohen Proceedings of the National Academy of Sciences 95 (12), 6909-6914, 1998 | 33 | 1998 |
| KRX autoinduction protocol: a convenient method for protein expression T Schagat, RF Ohana, P Otto, J Hartnett, M Slater Promega Notes 98, 16-18, 2008 | 23 | 2008 |
| Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer CW Hitko, T Kirkland, T Machleidt, RF Ohana, M Robers, K Wood US Patent 10,024,862, 2018 | 16 | 2018 |
| Chi-dependent intramolecular recombination in Escherichia coli R Friedman-Ohana, I Karunker, A Cohen Genetics 148 (2), 545-557, 1998 | 16 | 1998 |
