High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects BP Kleinstiver, V Pattanayak, MS Prew, SQ Tsai, NT Nguyen, Z Zheng, ... Nature 529 (7587), 490-495, 2016 | 2880 | 2016 |
Engineered CRISPR-Cas9 nucleases with altered PAM specificities BP Kleinstiver, MS Prew, SQ Tsai, VV Topkar, NT Nguyen, Z Zheng, ... Nature 523 (7561), 481-485, 2015 | 1914 | 2015 |
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy JS Chen, YS Dagdas, BP Kleinstiver, MM Welch, AA Sousa, ... Nature 550 (7676), 407-410, 2017 | 1165 | 2017 |
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants RT Walton, KA Christie, MN Whittaker, BP Kleinstiver Science 368 (6488), 290-296, 2020 | 959 | 2020 |
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition BP Kleinstiver, MS Prew, SQ Tsai, NT Nguyen, VV Topkar, Z Zheng, ... Nature Biotechnology, 2015 | 729 | 2015 |
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells BP Kleinstiver, SQ Tsai, MS Prew, NT Nguyen, MM Welch, JM Lopez, ... Nature Biotechnology 34 (8), 869-874, 2016 | 716 | 2016 |
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing BP Kleinstiver, AA Sousa, RT Walton, YE Tak, JY Hsu, K Clement, ... Nature biotechnology 37 (3), 276-282, 2019 | 581 | 2019 |
High levels of AAV vector integration into CRISPR-induced DNA breaks KS Hanlon, BP Kleinstiver, SP Garcia, MP Zaborowski, A Volak, SE Spirig, ... Nature communications 10 (1), 4439, 2019 | 340 | 2019 |
Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs J Listgarten, M Weinstein, BP Kleinstiver, AA Sousa, JK Joung, J Crawford, ... Nature biomedical engineering 2 (1), 38-47, 2018 | 289 | 2018 |
Discovery of widespread type I and type V CRISPR-Cas inhibitors ND Marino, JY Zhang, AL Borges, AA Sousa, LM Leon, BJ Rauch, ... Science 362 (6411), 240-242, 2018 | 248 | 2018 |
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize K Lee, Y Zhang, BP Kleinstiver, JA Guo, MJ Aryee, J Miller, A Malzahn, ... Plant biotechnology journal 17 (2), 362-372, 2019 | 228 | 2019 |
Inducible and multiplex gene regulation using CRISPR–Cpf1-based transcription factors YE Tak, BP Kleinstiver, JK Nuñez, JY Hsu, JE Horng, J Gong, ... Nature methods 14 (12), 1163-1166, 2017 | 208 | 2017 |
Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease CS McAlpine, J Park, A Griciuc, E Kim, SH Choi, Y Iwamoto, MG Kiss, ... Nature 595 (7869), 701-706, 2021 | 204 | 2021 |
Allele-specific gene editing prevents deafness in a model of dominant progressive hearing loss B György, C Nist-Lund, B Pan, Y Asai, KD Karavitaki, BP Kleinstiver, ... Nature medicine 25 (7), 1123-1130, 2019 | 185 | 2019 |
CRISPR/Cas9 Mediated Disruption of the SwedishAPP Allele as a Therapeutic Approach for Early-Onset Alzheimer’s Disease B György, C Lööv, MP Zaborowski, S Takeda, BP Kleinstiver, C Commins, ... Molecular Therapy-Nucleic Acids 11, 429-440, 2018 | 163 | 2018 |
Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma SK Saha, JD Gordan, BP Kleinstiver, P Vu, MS Najem, JC Yeo, L Shi, ... Cancer discovery 6 (7), 727-739, 2016 | 150 | 2016 |
Optimization of AsCas12a for combinatorial genetic screens in human cells PC DeWeirdt, KR Sanson, AK Sangree, M Hegde, RE Hanna, MN Feeley, ... Nature biotechnology 39 (1), 94-104, 2021 | 135 | 2021 |
Allele-Specific CRISPR-Cas9 Genome Editing of the Single-Base P23H Mutation for Rhodopsin-Associated Dominant Retinitis Pigmentosa P Li, BP Kleinstiver, MY Leon, MS Prew, D Navarro-Gomez, ... The CRISPR Journal 1 (1), 55-64, 2018 | 126 | 2018 |
Hypoxia drives transient site-specific copy gain and drug-resistant gene expression JC Black, E Atabakhsh, J Kim, KM Biette, C Van Rechem, B Ladd, ... Genes & development 29 (10), 1018-1031, 2015 | 106 | 2015 |
Engineered CRISPR-Cas9 nucleases JK Joung, B Kleinstiver, V Pattanayak US Patent 9,512,446, 2016 | 94 | 2016 |