Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element S Chong, FB Mersha, DG Comb, ME Scott, D Landry, LM Vence, ... Gene 192 (2), 271-281, 1997 | 918 | 1997 |
Semisynthesis of cytotoxic proteins using a modified protein splicing element TC Evans Jr, J Benner, MQ Xu Protein science 7 (11), 2256-2264, 1998 | 536 | 1998 |
Expanding the Genetic Code of Escherichia coli with Phosphoserine HS Park, MJ Hohn, T Umehara, LT Guo, EM Osborne, J Benner, CJ Noren, ... Science 333 (6046), 1151-1154, 2011 | 431 | 2011 |
Protein splicing involving the Saccharomyces cerevisiae VMA intein: the steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in … S Chong, Y Shao, H Paulus, J Benner, FB Perler, MQ Xu Journal of Biological Chemistry 271 (36), 22159-22168, 1996 | 373 | 1996 |
Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells PO Estève, HG Chin, J Benner, GR Feehery, M Samaranayake, ... Proceedings of the National Academy of Sciences 106 (13), 5076-5081, 2009 | 354 | 2009 |
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step S Chong, GE Montello, A Zhang, EJ Cantor, W Liao, MQ Xu, J Benner Nucleic acids research 26 (22), 5109-5115, 1998 | 325 | 1998 |
Characterization of a self-splicing mini-intein and its conversion into autocatalytic N-and C-terminal cleavage elements: facile production of protein building blocks for … S Mathys, TC Evans Jr, IC Chute, H Wu, S Chong, J Benner, XQ Liu, ... Gene 231 (1-2), 1-13, 1999 | 297 | 1999 |
Protein trans-Splicing and Cyclization by a Naturally Split Intein from the dnaE Gene ofSynechocystis Species PCC6803 TC Evans, D Martin, R Kolly, D Panne, L Sun, I Ghosh, L Chen, J Benner, ... Journal of Biological Chemistry 275 (13), 9091-9094, 2000 | 292 | 2000 |
Intervening sequences in an Archaea DNA polymerase gene. FB Perler, DG Comb, WE Jack, LS Moran, B Qiang, RB Kucera, J Benner, ... Proceedings of the National Academy of Sciences 89 (12), 5577-5581, 1992 | 289 | 1992 |
A Radically Different Mechanism for S-Adenosylmethionine–Dependent Methyltransferases TL Grove, JS Benner, MI Radle, JH Ahlum, BJ Landgraf, C Krebs, ... Science 332 (6029), 604-607, 2011 | 247 | 2011 |
The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum TC Evans, J Benner, MQ Xu Journal of Biological Chemistry 274 (7), 3923-3926, 1999 | 232 | 1999 |
Engineering Escherichia coli BL21 (DE3) derivative strains to minimize E. coli protein contamination after purification by immobilized metal affinity chromatography C Robichon, J Luo, TB Causey, JS Benner, JC Samuelson Applied and environmental microbiology 77 (13), 4634-4646, 2011 | 221 | 2011 |
The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins TC Evans, J Benner, MQ Xu Journal of Biological Chemistry 274 (26), 18359-18363, 1999 | 217 | 1999 |
Discrimination of methylcytosine from hydroxymethylcytosine in DNA molecules M Wanunu, D Cohen-Karni, RR Johnson, L Fields, J Benner, N Peterman, ... Journal of the American Chemical Society 133 (3), 486-492, 2011 | 212 | 2011 |
Substrate specificity and kinetic mechanism of mammalian G9a histone H3 methyltransferase D Patnaik, HG Chin, PO Esteve, J Benner, SE Jacobsen, S Pradhan Journal of Biological Chemistry 279 (51), 53248-53258, 2004 | 184 | 2004 |
Automethylation of G9a and its implication in wider substrate specificity and HP1 binding HG Chin, PO Esteve, M Pradhan, J Benner, D Patnaik, MF Carey, ... Nucleic acids research 35 (21), 7313-7323, 2007 | 153 | 2007 |
Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase S Pradhan, D Talbot, M Sha, J Benner, L Hornstra, E Li, R Jaenisch, ... Nucleic acids research 25 (22), 4666-4673, 1997 | 146 | 1997 |
Thermostable Bst DNA polymerase I lacks a 3′→ 5′ proofreading exonuclease activity JM Aliotta, JJ Pelletier, JL Ware, LS Moran, JS Benner, H Kong Genetic analysis: biomolecular engineering 12 (5-6), 185-195, 1996 | 146 | 1996 |
Characterization and expression of the Escherichia coli Mrr restriction system PA Waite-Rees, CJ Keating, LS Moran, BE Slatko, LJ Hornstra, JS Benner Journal of bacteriology 173 (16), 5207-5219, 1991 | 146 | 1991 |
An alternative protein splicing mechanism for inteins lacking an N‐terminal nucleophile MW Southworth, J Benner, FB Perler The EMBO journal, 2000 | 144 | 2000 |