Large-scale purification of an antibody directed against hepatitis B surface antigen from transgenic tobacco plants R Valdés, L Gómez, S Padilla, J Brito, B Reyes, T Alvarez, O Mendoza, ... Biochemical and biophysical research communications 308 (1), 94-100, 2003 | 119 | 2003 |
Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale R Valdés, B Reyes, T Alvarez, J Garcı́a, JA Montero, A Figueroa, L Gómez, ... Biochemical and biophysical research communications 310 (3), 742-747, 2003 | 51 | 2003 |
A rapid and sensitive ELISA to quantify an HBsAg specific monoclonal antibody and a plant-derived antibody during their downstream purification process A Leyva, A Franco, T González, JC Sánchez, I López, D Geada, ... Biologicals 35 (1), 19-25, 2007 | 29 | 2007 |
Detection of Rubisco and mycotoxins as potential contaminants of a plantibody against the hepatitis B surface antigen purified from tobacco D Geada, R Valdés, A Escobar, DM Ares, E Torres, R Blanco, W Ferro, ... Biologicals 35 (4), 309-315, 2007 | 9 | 2007 |
A summary of the manufacture, biochemical characterization, and virological safety demonstration of the mouse mAb CB. Hep-1 used to produce the hepatitis B vaccine R Valdés, A Tamayo, M González, L Dorta, J Montero, A Figueroa Bioprocess J 8 (3), 13-31, 2009 | 7 | 2009 |
La simbiosis micorrízica arbuscular en plantas de arroz (Oryza sativa L.) sometidas a estrés hídrico. Parte II Respuesta bioquímica M Ruiz-Sánchez, D Geada, Y Muñoz Hernández, A Martínez, Y Santana, ... Cultivos Tropicales 36 (3), 88-95, 2015 | 6 | 2015 |
Assessment of a plantibody HB-01 purification strategy at different scales S Padilla, R Valdés, L Gómez, D Geada, W Ferro, O Mendoza, C García, ... Chromatographia 70 (11), 1673-1678, 2009 | 6 | 2009 |
Mouse Hybridoma Cell Culture in a Protein-Free Medium Using a Bio-Mimicking Fish-Tail Disc Stirred Bioreactor. R Valdés, H Aragón, M González, D Hernández, D Geada, D Goitizolo, ... BioProcessing Journal 16 (1), 2017 | 5 | 2017 |
Production of a monoclonal antibody by ascites, hollow fiber system, and transgenic plants for vaccine production using CB. Hep-1 mAb as a study case R Valdés, A Tamayo, M González, S Padilla, D Geada, W Ferro, L Milá, ... Biotechnology and Bioprocess Engineering 17 (1), 145-159, 2012 | 5 | 2012 |
New Mab CB. Hep-1 purification process eliminates the need for pre-chromatographic purification. Stability demonstrated over 100 purification cycles R Valdés, T Álvarez, A Tamayo, EG Fernandez, J Montero, D Geada, ... Chromatographia 67 (11), 923-927, 2008 | 5 | 2008 |
Comparison of different ligand densities in immunoaffinity chromatography of the plantibody HB-01 coupled to Sepharose CL-4B to purify the rHBsAg R Valdés, Y Medina, W Ferro, B Reyes, D Geada, J Montero, T Alvarez, ... Journal of Chromatography B 852 (1-2), 1-7, 2007 | 5 | 2007 |
Assessment of a protein-free medium performance in dfferent cell culture vessels using mouse hybridomas to produce monoclonal antibodies R Valdés, M González, D Geada, E Fernández Pharmaceut Anal Acta 3 (4), 2012 | 4 | 2012 |
Using quality risk management in the plantibody HB-01 manufacturing by transgenic tobacco plants for vaccine production L Mila, R Valdes, S Padilla, O Mendoza, L Gomez, C García, D Geada, ... Latin American Journal of Pharmacy 29, 2010 | 4 | 2010 |
Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use R Valdes, Y Medina, EG Fernández, D Geada, W Ferro, T Álvarez, ... Latin American Journal of Pharmacy 27, 2008 | 4 | 2008 |
Mycorrhizae arbuscular symbiosis in rice plants (Oryza sativa L.) under water stress. Part II: Biochemical response. M Ruiz-Sánchez, D Geada, Y Muñoz Hernández, A Martínez, Y Santana, ... Cultivos Tropicales 36 (3), 88-95, 2015 | 3 | 2015 |
Influence of the number of animals on the production of monoclonal antibody CB. Hep-1 by the Ascites method O Mendoza, R Valdés, M González, R Alemán, T Álvarez, S Padilla, ... Biotecnología Aplicada 26 (1), 26, 2009 | 3 | 2009 |
Two-Step Purification of Antibody from Tobacco Plants for Vaccine Manufacturing: Aqueous Two-Phase Extraction and Affinity Chromatography. W FERRO, T ÁLVAREZ, D GEADA, Y MEDINA, Y GUEVARA, ... BioProcessing Journal 14 (1), 2015 | 2 | 2015 |
Kinetic of expression of a plantibody in (Nicotiana tabacum) plants cultivated in different substrates (Zeolite and Soil) L Gomez, R Valdes, M Pujol, S Padilla, T Gonzalez, M Font, W Ferro, ... Journal of Agronomy, 2011 | 2 | 2011 |
Assessment of a N. tabacum L., variety using natural zeolite as substrate and confined conditions for consistent biomass, protein and plantibody production. O Mendoza, R Valdes, S Padilla, Y Issac, D Gavilan, Y Lescaille, Y Avila, ... Journal of Agronomy 10 (3), 74-83, 2011 | 2 | 2011 |
Significant improvements in the performance of an established affinity chromatography procedure employed to purify a monoclonal antibody in 100 purification cycles W Ferro, R Valdés, E Fernández, Y Guevara, Y Medina, T Alvarez, ... Pharm. Anal. Acta 2, 2-7, 2011 | 2 | 2011 |