Transcription factor‐based biosensors in high‐throughput screening: advances and applications F Cheng, XL Tang, T Kardashliev Biotechnology journal 13 (7), 1700648, 2018 | 90 | 2018 |
Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations J Zhao, T Kardashliev, A Joëlle Ruff, M Bocola, U Schwaneberg Biotechnology and bioengineering 111 (12), 2380-2389, 2014 | 61 | 2014 |
An Enzymatic Route to α‐Tocopherol Synthons: Aromatic Hydroxylation of Pseudocumene and Mesitylene with P450 BM3 A Dennig, AM Weingartner, T Kardashliev, CA Müller, E Tassano, ... Chemistry–A European Journal 23 (71), 17981-17991, 2017 | 41 | 2017 |
A competitive flow cytometry screening system for directed evolution of therapeutic enzyme F Cheng, T Kardashliev, C Pitzler, A Shehzad, H Lue, J Bernhagen, L Zhu, ... ACS synthetic biology 4 (7), 768-775, 2015 | 41 | 2015 |
An engineered outer membrane pore enables an efficient oxygenation of aromatics and terpenes AJ Ruff, M Arlt, M van Ohlen, T Kardashliev, M Konarzycka-Bessler, ... Journal of Molecular Catalysis B: Enzymatic 134, 285-294, 2016 | 26 | 2016 |
A Semi‐Rationally Engineered Bacterial Pyrrolysyl‐tRNA Synthetase Genetically Encodes Phenyl Azide Chemistry P Fladischer, A Weingartner, J Blamauer, B Darnhofer, ... Biotechnology Journal 14 (3), 1800125, 2019 | 16 | 2019 |
Directed evolution of a surface-displayed artificial allylic deallylase relying on a GFP reporter protein A Baiyoumy, J Vallapurackal, F Schwizer, T Heinisch, T Kardashliev, ... ACS catalysis 11 (17), 10705-10712, 2021 | 14 | 2021 |
A high-throughput screening method to reengineer DNA polymerases for random mutagenesis T Kardashliev, AJ Ruff, J Zhao, U Schwaneberg Molecular biotechnology 56, 274-283, 2014 | 13 | 2014 |
The sequence saturation mutagenesis (SeSaM) method AJ Ruff, T Kardashliev, A Dennig, U Schwaneberg Directed evolution library creation: Methods and protocols, 45-68, 2014 | 13 | 2014 |
Whole-cell screening of oxidative enzymes using genetically encoded sensors T Kardashliev, A Weingartner, E Romero, U Schwaneberg, M Fraaije, ... Chemical Science 12 (44), 14766-14772, 2021 | 10 | 2021 |
Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations J Zhao, VJ Frauenkron-Machedjou, T Kardashliev, AJ Ruff, L Zhu, ... Applied microbiology and biotechnology 101, 3177-3187, 2017 | 10 | 2017 |
Efficient synthesis of 2,6-bis(hydroxymethyl)pyridine using whole-cell biocatalysis T Kardashliev, S Panke, M Held Green Chemistry, 2022 | 3 | 2022 |
Novel sensors for engineering microbiology MO Bahls, T Kardashliev, S Panke Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids …, 2017 | 3 | 2017 |
Biotin-Independent Saccharomyces cerevisiae with Enhanced Growth: Engineering an Acetyl-CoA Carboxylase Bypass M Slanska, SA Haider, T Kardashliev, D Huck, CC Liu, TR Ward bioRxiv, 2024.10. 04.616611, 2024 | | 2024 |
Monooxygenase mutants for biosynthesis of 2,6-bis(hydroxymethyl)pyridine and a method for preparation of 2,6-bis(hydroxymethyl)pyridine using the said monooxygenase mutants T Kardashliev, S Panke, M Held | | 2023 |
Method for preparation of 2,6-bis(hydroxymethyl)pyridine via enzymatic catalysis Tsvetan Kardashliev, Sven Panke, Martin Held WO Patent WO2022008628A1, 2022 | | 2022 |
Assay for screening of recombinant cells and microbes Tsvetan Kardashliev, Jacqueline Mock, Sven Panke, Martin Held WO Patent WO2020089235A1, 2020 | | 2020 |
Directed Evolution of DNA Polymerases for Advancement of the SeSaM Mutagenesis Method and Biotransformations with P450 BM3 Monooxygenase TD Kardashliev Dissertation, Aachen, Techn. Hochsch., 2015, 2015 | | 2015 |