Cell-free gene expression: an expanded repertoire of applications AD Silverman, AS Karim, MC Jewett Nature Reviews Genetics 21 (3), 151-170, 2020 | 473 | 2020 |
Metabolic engineering of muconic acid production in Saccharomyces cerevisiae KA Curran, JM Leavitt, AS Karim, HS Alper Metabolic engineering 15, 55-66, 2013 | 344 | 2013 |
Cell‐free metabolic engineering: biomanufacturing beyond the cell QM Dudley, AS Karim, MC Jewett Biotechnology journal 10 (1), 69-82, 2015 | 336 | 2015 |
Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale FE Liew, R Nogle, T Abdalla, BJ Rasor, C Canter, RO Jensen, L Wang, ... Nature biotechnology 40 (3), 335-344, 2022 | 275 | 2022 |
Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications KA Curran, AS Karim, A Gupta, HS Alper Metabolic engineering 19, 88-97, 2013 | 251 | 2013 |
Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications AS Karim, KA Curran, HS Alper FEMS yeast research 13 (1), 107-116, 2013 | 251 | 2013 |
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery AS Karim, MC Jewett Metabolic engineering 36, 116-126, 2016 | 234 | 2016 |
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design AS Karim, QM Dudley, A Juminaga, Y Yuan, SA Crowe, JT Heggestad, ... Nature Chemical Biology 16, 912-919, 2020 | 185 | 2020 |
Design of synthetic yeast promoters via tuning of nucleosome architecture KA Curran, NC Crook, AS Karim, A Gupta, AM Wagman, HS Alper Nature communications 5 (1), 4002, 2014 | 162 | 2014 |
A pressure test to make 10 molecules in 90 days: external evaluation of methods to engineer biology A Casini, FY Chang, R Eluere, AM King, EM Young, QM Dudley, A Karim, ... Journal of the American Chemical Society 140 (12), 4302-4316, 2018 | 151 | 2018 |
In vitro prototyping of limonene biosynthesis using cell-free protein synthesis QM Dudley, AS Karim, CJ Nash, MC Jewett Metabolic Engineering 61, 251-260, 2020 | 100 | 2020 |
Controlling cell-free metabolism through physiochemical perturbations AS Karim, JT Heggestad, SA Crowe, MC Jewett Metabolic engineering 45, 86-94, 2018 | 77 | 2018 |
Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin L Zhuang, S Huang, WQ Liu, AS Karim, MC Jewett, J Li Metabolic Engineering 60, 37-44, 2020 | 68 | 2020 |
Cell-free synthetic biology for pathway prototyping AS Karim, MC Jewett Methods in enzymology 608, 31-57, 2018 | 64 | 2018 |
Toward sustainable, cell-free biomanufacturing BJ Rasor, B Vögeli, GM Landwehr, JW Bogart, AS Karim, MC Jewett Current Opinion in Biotechnology 69, 136-144, 2021 | 63 | 2021 |
Cell-free styrene biosynthesis at high titers WS Grubbe, BJ Rasor, A Krüger, MC Jewett, AS Karim Metabolic Engineering 61, 89-95, 2020 | 55 | 2020 |
A low-cost, thermostable, cell-free protein synthesis platform for on-demand production of conjugate vaccines KF Warfel, A Williams, DA Wong, SE Sobol, P Desai, J Li, YF Chang, ... ACS synthetic biology 12 (1), 95-107, 2022 | 42 | 2022 |
High-throughput optimization cycle of a cell-free ribosome assembly and protein synthesis system F Caschera, AS Karim, G Gazzola, AE d’Aquino, NH Packard, MC Jewett ACS synthetic biology 7 (12), 2841-2853, 2018 | 42 | 2018 |
Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria B Vögeli, L Schulz, S Garg, K Tarasava, JM Clomburg, SH Lee, A Gonnot, ... Nature communications 13 (1), 3058, 2022 | 40 | 2022 |
Cell‐free exploration of the natural product chemical space JW Bogart, MD Cabezas, B Vögeli, DA Wong, AS Karim, MC Jewett ChemBioChem 22 (1), 84-91, 2021 | 39 | 2021 |