关注
Alexander A Sousa
Alexander A Sousa
PhD Candidate, Harvard Medical School
在 g.harvard.edu 的电子邮件经过验证
标题
引用次数
引用次数
年份
Search-and-replace genome editing without double-strand breaks or donor DNA
AV Anzalone, PB Randolph, JR Davis, AA Sousa, LW Koblan, JM Levy, ...
Nature 576 (7785), 149-157, 2019
36312019
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
JS Chen, YS Dagdas, BP Kleinstiver, MM Welch, AA Sousa, ...
Nature 550 (7676), 407-410, 2017
11572017
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
BP Kleinstiver, AA Sousa, RT Walton, YE Tak, JY Hsu, K Clement, ...
Nature biotechnology 37 (3), 276-282, 2019
5682019
Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications
AE Friedland, R Baral, P Singhal, K Loveluck, S Shen, M Sanchez, ...
Genome biology 16, 1-10, 2015
3452015
High levels of AAV vector integration into CRISPR-induced DNA breaks
KS Hanlon, BP Kleinstiver, SP Garcia, MP Zaborowski, A Volak, SE Spirig, ...
Nature communications 10 (1), 4439, 2019
3362019
Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs
J Listgarten, M Weinstein, BP Kleinstiver, AA Sousa, JK Joung, J Crawford, ...
Nature biomedical engineering 2 (1), 38-47, 2018
2842018
Discovery of widespread type I and type V CRISPR-Cas inhibitors
ND Marino, JY Zhang, AL Borges, AA Sousa, LM Leon, BJ Rauch, ...
Science 362 (6411), 240-242, 2018
2442018
Designing and executing prime editing experiments in mammalian cells
JL Doman, AA Sousa, PB Randolph, PJ Chen, DR Liu
Nature protocols 17 (11), 2431-2468, 2022
592022
Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice
KA Everette, GA Newby, RM Levine, K Mayberry, Y Jang, T Mayuranathan, ...
Nature biomedical engineering 7 (5), 616-628, 2023
512023
Adenine transversion editors enable precise, efficient A• T-to-C• G base editing in mammalian cells and embryos
L Chen, M Hong, C Luan, H Gao, G Ru, X Guo, D Zhang, S Zhang, C Li, ...
Nature biotechnology 42 (4), 638-650, 2024
492024
Variants of Cpf1 (Cas12a) with altered PAM specificity
JK Joung, B Kleinstiver, A Sousa
US Patent 11,591,589, 2023
402023
Enhanced Proofreading Governs CRISPR-Cas9 Targeting Accuracy
JS Chen, YS Dagdas, BP Kleinstiver, MM Welch, AA Sousa, ...
Biophysical Journal 114 (3), 194a, 2018
92018
Publisher Correction: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
BP Kleinstiver, AA Sousa, RT Walton, YE Tak, JY Hsu, K Clement, ...
Nature biotechnology 38 (7), 901, 2020
42020
Variants of cpf1 (cas12a) with altered pam specificity
JK Joung, B KLEINSTIVER, A SOUSA
42018
Systematic optimization of prime editing for the efficient functional correction of CFTR F508del in human airway epithelial cells
AA Sousa, C Hemez, L Lei, S Traore, K Kulhankova, GA Newby, ...
Nature biomedical engineering, 1-15, 2024
2024
Enhancement and Application of Prime Editing to Correct Pathogenic Mutations In Vitro and In Vivo
AA Sousa
2024
Development of a Prime Editing Strategy to Treat Mutations in the Crumbs Homologue 1 (CRB1) Gene
BL Costa, AA Sousa, YT Tsai, Y Li, IH Maumenee, DR Liu, SH Tsang, ...
Investigative Ophthalmology & Visual Science 64 (8), 3862-3862, 2023
2023
Development of a Prime Editing Strategy to Treat Mutations in the Crumbs Homologue 1 (CRB1) Gene
BL Da Costa, AA Sousa, YT Tsai, Y Li, IH Maumenee, DR Liu, SH Tsang, ...
MOLECULAR THERAPY 30 (4), 260-261, 2022
2022
Variants of CPF1 (CAS12a) With Altered PAM Specificity
JK Joung, B Kleinstiver, A Sousa
US Patent App. 17/244,738, 2021
2021
Enhancing Genome Editing with Engineered CRISPR Nucleases
RT Walton, AA Sousa, MM Welch, YE Tak, JY Hsu, K Clement, JE Horng, ...
MOLECULAR THERAPY 27 (4), 104-105, 2019
2019
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