| Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria U Brockmeier, M Caspers, R Freudl, A Jockwer, T Noll, T Eggert Journal of molecular biology 362 (3), 393-402, 2006 | 349 | 2006 |
| Translocation of proteins across the cell envelope of Gram-positive bacteria KHM van Wely, J Swaving, R Freudl, AJM Driessen FEMS microbiology reviews 25 (4), 437-454, 2001 | 325 | 2001 |
| Signal peptides for recombinant protein secretion in bacterial expression systems R Freudl Microbial cell factories 17 (1), 52, 2018 | 269 | 2018 |
| Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12 R Freudl, S MacIntyre, M Degen, U Henning Journal of molecular biology 188 (3), 491-494, 1986 | 195 | 1986 |
| An outer membrane protein (OmpA) of Escherichia coli K-12 undergoes a conformational change during export. R Freudl, H Schwarz, YD Stierhof, K Gamon, I Hindennach, U Henning Journal of Biological Chemistry 261 (24), 11355-11361, 1986 | 166 | 1986 |
| An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform P Rohe, D Venkanna, B Kleine, R Freudl, M Oldiges Microbial Cell Factories 11, 1-14, 2012 | 140 | 2012 |
| The efficient export of NADP‐containing glucose‐fructose oxidoreductase to the periplasm of Zymomonas mobilis depends both on an intact twin‐arginine motif in … D Halbig, T Wiegert, N Blaudeck, R Freudl, GA Sprenger European journal of biochemistry 263 (2), 543-551, 1999 | 134 | 1999 |
| The LysE Superfamily: Topology of the Lysine Exporter LysE of Corynebacterium glutamicum, a Paradyme for a Novel Superfamily of Transmembrane Solute Translocators MHSJ M Vrljic, J Garg, A Bellmann, S Wachi, R Freudl, MJ Malecki, H Sahm, VJ ... Journal of molecular microbiology and biotechnology 1 (2), 327-336, 1999 | 122 | 1999 |
| Isolation and characterization of bifunctional Escherichia coli TatA mutant proteins that allow efficient Tat-dependent protein translocation in the absence of TatB N Blaudeck, P Kreutzenbeck, M Muller, GA Sprenger, R Freudl Journal of Biological Chemistry 280 (5), 3426-3432, 2005 | 117 | 2005 |
| The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K‐12. R Freudl, H Schwarz, M Klose, NR Movva, U Henning The EMBO Journal 4 (13A), 3593-3598, 1985 | 115 | 1985 |
| Genetic Analysis of Pathway Specificity during Posttranslational Protein Translocation across the Escherichia coli Plasma Membrane N Blaudeck, P Kreutzenbeck, R Freudl, GA Sprenger Journal of bacteriology 185 (9), 2811-2819, 2003 | 108 | 2003 |
| Signal peptide peptidase-and ClpP-like proteins of Bacillus subtilis required for efficient translocation and processing of secretory proteins A Bolhuis, A Matzen, HL Hyyryläinen, VP Kontinen, R Meima, J Chapuis, ... Journal of Biological Chemistry 274 (35), 24585-24592, 1999 | 100 | 1999 |
| Specificity of signal peptide recognition in Tat-dependent bacterial protein translocation N Blaudeck, GA Sprenger, R Freudl, T Wiegert Journal of bacteriology 183 (2), 604-610, 2001 | 99 | 2001 |
| Improvement of Sec-dependent secretion of a heterologous model protein in Bacillus subtilis by saturation mutagenesis of the N-domain of the AmyE signal peptide M Caspers, U Brockmeier, C Degering, T Eggert, R Freudl Applied microbiology and biotechnology 86, 1877-1885, 2010 | 98 | 2010 |
| Comparative analysis of twin-arginine (Tat)-dependent protein secretion of a heterologous model protein (GFP) in three different Gram-positive bacteria D Meissner, A Vollstedt, JM Van Dijl, R Freudl Applied microbiology and biotechnology 76, 633-642, 2007 | 95 | 2007 |
| Characterization of a Bacillus subtilis SecA mutant protein deficient in translocation ATPase and release from the membrane J Van der Wolk, M Klose, E Breukink, RA Demel, B De Kruijff, R Freudl, ... Molecular microbiology 8 (1), 31-42, 1993 | 93 | 1993 |
| Escherichia coli twin arginine (Tat) mutant translocases possessing relaxed signal peptide recognition specificities P Kreutzenbeck, C Kroöger, F Lausberg, N Blaudeck, GA Sprenger, ... Journal of Biological Chemistry 282 (11), 7903-7911, 2007 | 87 | 2007 |
| Transmembrane insertion of twin-arginine signal peptides is driven by TatC and regulated by TatB J Fröbel, P Rose, F Lausberg, AS Blümmel, R Freudl, M Müller Nature communications 3 (1), 1311, 2012 | 86 | 2012 |
| Internal deletions in the gene for an Escherichia coli outer membrane protein define an area possibly important for recognition of the outer membrane by this polypeptide. M Klose, H Schwarz, S MacIntyre, R Freudl, ML Eschbach, U Henning Journal of Biological Chemistry 263 (26), 13291-13296, 1988 | 86 | 1988 |
| Insertion of peptides into cell-surface-exposed areas of the Escherichia coli OmpA protein does not interfere with export and membrane assembly R Freudl Gene 82 (2), 229-236, 1989 | 80 | 1989 |
