Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos, Á Berenguer-Murcia, ... Biotechnology advances 37 (5), 746-770, 2019 | 505 | 2019 |
Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts JJ Virgen-Ortiz, JCS Dos Santos, Á Berenguer-Murcia, O Barbosa, ... Journal of materials chemistry B 5 (36), 7461-7490, 2017 | 272 | 2017 |
Effect of protein load on stability of immobilized enzymes L Fernandez-Lopez, SG Pedrero, N Lopez-Carrobles, BC Gorines, ... Enzyme and microbial technology 98, 18-25, 2017 | 208 | 2017 |
Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil: Critical role of the immobilization support VG Tacias-Pascacio, JJ Virgen-Ortíz, M Jiménez-Pérez, M Yates, ... Fuel 200, 1-10, 2017 | 139 | 2017 |
Effect of immobilization rate and enzyme crowding on enzyme stability under different conditions. The case of lipase from Thermomyces lanuginosus immobilized on octyl agarose beads H Zaak, EH Siar, JF Kornecki, L Fernandez-Lopez, SG Pedrero, ... Process Biochemistry 56, 117-123, 2017 | 130 | 2017 |
Relevance of substrates and products on the desorption of lipases physically adsorbed on hydrophobic supports JJ Virgen-Ortíz, VG Tacias-Pascacio, DB Hirata, B Torrestiana-Sanchez, ... Enzyme and Microbial Technology 96, 30-35, 2017 | 126 | 2017 |
Comparison of acid, basic and enzymatic catalysis on the production of biodiesel after RSM optimization VG Tacias-Pascacio, B Torrestiana-Sánchez, L Dal Magro, JJ Virgen-Ortíz, ... Renewable Energy 135, 1-9, 2019 | 109 | 2019 |
Biotechnological relevance of the lipase A from Candida antarctica RRC Monteiro, JJ Virgen-Ortiz, A Berenguer-Murcia, TN da Rocha, ... Catalysis Today 362, 141-154, 2021 | 104 | 2021 |
Evaluation of different commercial hydrophobic supports for the immobilization of lipases: Tuning their stability, activity and specificity VG Tacias-Pascacio, S Peirce, B Torrestiana-Sanchez, M Yates, ... RSC advances 6 (102), 100281-100294, 2016 | 92 | 2016 |
Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase S Peirce, JJ Virgen-Ortíz, VG Tacias-Pascacio, N Rueda, ... RSC advances 6 (66), 61707-61715, 2016 | 90 | 2016 |
Evaluation of different immobilized lipases in transesterification reactions using tributyrin: Advantages of the heterofunctional octyl agarose beads DB Hirata, TL Albuquerque, N Rueda, JJ Virgen-Ortíz, ... Journal of Molecular Catalysis B: Enzymatic 133, 117-123, 2016 | 78 | 2016 |
Lecitase ultra: A phospholipase with great potential in biocatalysis JJ Virgen-Ortiz, JCS dos Santos, C Ortiz, A Berenguer-Murcia, O Barbosa, ... Molecular Catalysis 473, 110405, 2019 | 76 | 2019 |
Genipin as an emergent tool in the design of biocatalysts: mechanism of reaction and applications VG Tacias-Pascacio, E García-Parra, G Vela-Gutiérrez, JJ Virgen-Ortiz, ... Catalysts 9 (12), 1035, 2019 | 72 | 2019 |
Physical crosslinking of lipase from Rhizomucor miehei immobilized on octyl agarose via coating with ionic polymers: Avoiding enzyme release from the support L Fernandez-Lopez, SG Pedrero, N Lopez-Carrobles, JJ Virgen-Ortíz, ... Process Biochemistry 54, 81-88, 2017 | 66 | 2017 |
Desorption of lipases immobilized on octyl-agarose beads and coated with ionic polymers after thermal inactivation. Stronger adsorption of polymers/unfolded protein composites JJ Virgen-Ortíz, SG Pedrero, L Fernandez-Lopez, N Lopez-Carrobles, ... Molecules 22 (1), 91, 2017 | 61 | 2017 |
Reuse of anion exchangers as supports for enzyme immobilization: Reinforcement of the enzyme-support multiinteraction after enzyme inactivation JJ Virgen-Ortíz, S Peirce, VG Tacias-Pascacio, V Cortes-Corberan, ... Process Biochemistry 51 (10), 1391-1396, 2016 | 55 | 2016 |
Stabilization of Candida antarctica Lipase B (CALB) Immobilized on Octyl Agarose by Treatment with Polyethyleneimine (PEI) S Peirce, VG Tacias-Pascacio, ME Russo, A Marzocchella, JJ Virgen-Ortíz, ... Molecules 21 (6), 751, 2016 | 54 | 2016 |
Comparison of nutritional properties and bioactive compounds between industrial and artisan fresh tortillas from maize landraces C Colín-Chávez, JJ Virgen-Ortiz, LE Serrano-Rubio, MA Martínez-Téllez, ... Current Research in Food Science 3, 189-194, 2020 | 51 | 2020 |
Method to concentrate protein solutions based on dialysis–freezing–centrifugation: Enzyme applications JJ Virgen-Ortíz, V Ibarra-Junquera, JA Osuna-Castro, ... Analytical Biochemistry 426 (1), 4-12, 2012 | 47 | 2012 |
Optimization of the coating of octyl-CALB with ionic polymers to improve stability and decrease enzyme leakage L Fernandez-Lopez, JJ Virgen-OrtÍz, SG Pedrero, N Lopez-Carrobles, ... Biocatalysis and Biotransformation 36 (1), 47-56, 2018 | 45 | 2018 |