Current and prospective applications of metal ion–protein binding EKM Ueda, PW Gout, L Morganti Journal of chromatography A 988 (1), 1-23, 2003 | 589 | 2003 |
High-yield purification of biosynthetic human growth hormone secreted in Escherichia coli periplasmic space JE de Oliveira, CRJ Soares, CN Peroni, E Gimbo, IMC Camargo, ... Journal of Chromatography A 852 (2), 441-450, 1999 | 80 | 1999 |
High‐level expression of human thyroid‐stimulating hormone in Chinese hamster ovary cells by co‐transfection of dicistronic expression vectors followed by a dual‐marker … CN Peroni, CRJ Soares, E Gimbo, L Morganti, MTCP Ribela, P Bartolini Biotechnology and Applied Biochemistry 35 (1), 19-26, 2002 | 56 | 2002 |
VapC from the leptospiral VapBC toxin-antitoxin module displays ribonuclease activity on the initiator tRNA APY Lopes, LM Lopes, TR Fraga, RM Chura-Chambi, AL Sanson, ... PloS one 9 (7), e101678, 2014 | 49 | 2014 |
Refolding of endostatin from inclusion bodies using high hydrostatic pressure RM Chura-Chambi, LA Genova, R Affonso, L Morganti Analytical Biochemistry 379 (1), 32-39, 2008 | 49 | 2008 |
Ni (II)-based immobilized metal ion affinity chromatography of recombinant human prolactin from periplasmic Escherichia coli extracts EKM Ueda, PW Gout, L Morganti Journal of chromatography A 922 (1-2), 165-175, 2001 | 43 | 2001 |
High‐level synthesis of human prolactin in Chinese‐hamster ovary cells CRJ Soares, L Morganti, B Miloux, JH Lupker, P Ferrara, P Bartolini Biotechnology and Applied Biochemistry 32 (2), 127-135, 2000 | 40 | 2000 |
Protein refolding at high pressure: optimization using eGFP as a model NV Malavasi, D Foguel, CFS Bonafe, C Braga, RM Chura-Chambi, ... Process Biochemistry 46 (2), 512-518, 2011 | 38 | 2011 |
Reversed-phase high-performance liquid chromatography method for the determination of prolactin in bacterial extracts and in its purified form CRJ Soares, IMC Camargo, L Morganti, E Gimbo, JE de Oliveira, ... Journal of Chromatography A 955 (2), 229-236, 2002 | 38 | 2002 |
Cloning of serine protease cDNAs from Crotalus durissus terrificus venom gland and expression of a functional Gyroxin homologue in COS-7 cells CM Yonamine, ARB Prieto-da-Silva, GS Magalhães, G Rádis-Baptista, ... Toxicon 54 (2), 110-120, 2009 | 37 | 2009 |
Anti‐tumor effect of endostatin mediated by retroviral gene transfer in mice bearing renal cell carcinoma EL Coutinho, LN de Sousa Andrade, R Chammas, L Morganti, N Schor, ... The FASEB Journal 21 (12), 3153-3161, 2007 | 35 | 2007 |
An analysis of the factors that affect the dissociation of inclusion bodies and the refolding of endostatin under high pressure RM Chura-Chambi, Y Cordeiro, NV Malavasi, LS Lemke, D Rodrigues, ... Process biochemistry 48 (2), 250-259, 2013 | 32 | 2013 |
Synthesis and characterization of recombinant, authentic human prolactin secreted into the periplasmic space of Escherichia coli L Morganti, CRJ Soares, R Affonso, PW Gout, P Bartolini Biotechnology and applied biochemistry 27 (1), 63-70, 1998 | 29 | 1998 |
Production and characterization of biologically active Ala‐Ser‐(His) 6‐Ile‐Glu‐Gly‐Arg‐human prolactin (tag‐hPRL) secreted in the periplasmic space of Escherichia coli L Morganti, M Huyer, G PW, P Bartolini Biotechnology and applied biochemistry 23 (1), 67-75, 1996 | 29 | 1996 |
The use of recombinant human growth hormone for radioiodination and standard preparation in radioimmunoassay MTCP Ribela, Y Murata, L Morganti, D Toniolo, P Bartolini Journal of immunological methods 159 (1-2), 269-274, 1993 | 28 | 1993 |
Refolding of the recombinant protein OmpA70 from Leptospira interrogans from inclusion bodies using high hydrostatic pressure and partial characterization of its immunological … TR Fraga, RM Chura-Chambi, AP Gonçales, ZM Morais, SA Vasconcellos, ... Journal of biotechnology 148 (2-3), 156-162, 2010 | 27 | 2010 |
Protein refolding based on high hydrostatic pressure and alkaline pH: Application on a recombinant dengue virus NS1 protein RM Chura-Chambi, CMR da Silva, LR Pereira, P Bartolini, LCS Ferreira, ... PLoS One 14 (1), e0211162, 2019 | 23 | 2019 |
Generation of a Chinese hamster ovary cell line producing recombinant human glucocerebrosidase JB Novo, L Morganti, AM Moro, AF Paes Leme, SMT Serrano, I Raw, ... BioMed Research International 2012 (1), 875383, 2012 | 21 | 2012 |
Anti-tumor therapy with macroencapsulated endostatin producer cells DB Rodrigues, R Chammas, NV Malavasi, PLN da Costa, ... BMC biotechnology 10, 1-9, 2010 | 18 | 2010 |
Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus CM Rosa da Silva, RM Chura-Chambi, L Ramos Pereira, Y Cordeiro, ... BMC biotechnology 18, 1-10, 2018 | 17* | 2018 |