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The aim of this study was to examine antioxidant and inhibitory activity of Gly‐Ala‐Ser‐Arg‐His‐Trp‐Tyr‐Phe‐Leu peptide named GL‐9 peptide. The synthetic GL‐9 peptide was purified by C₈ reveres phase HPLC (RP‐HPLC). The antioxidant activity of the GL‐9 peptide was investigated based on its scavenging capacity1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical, 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulphonic acid) diammonium salt (ABTS), superoxide ( ), hydroxyl (OH^•−) and lipid peroxidation inhibition. The ACE inhibitory activity and kinetic parameters of the peptide were determined using FAPPG as substrate. GL‐9 peptide/ACE complex interaction, position, type and energy was also investigated by using molecular docking. GL‐9 peptide displays DPPH (IC₅₀ = 6.5 µg/mL), ABTS•⁺ (IC₅₀ = 7.2 µg/mL), superoxide (IC₅₀ = 122 µg/mL) and hydroxyl (IC₅₀ = 60.0 µg/mL) radical scavengers. GL‐9 …