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Green coffee oil (GCO) has been used in cosmetic formulations due to its emollient and anti‐ageing properties. However, there are insufficient studies about its safety when applied in cosmetic formulations.

Cytotoxicity of GCO and of formulations containing 2.5–15% of GCO was evaluated by the MTT reduction assay, in human keratinocytes. Formulations containing 15% of GCO and the vehicle were applied under in use conditions in the volar forearm of human volunteers during 3 days. Transepidermal water loss, stratum corneum water content and erythema index were evaluated each 24 h using biophysical techniques. The same formulations were probed for skin tolerance through a patch test.

Neither pure GCO nor its formulations showed cytotoxic effects in concentrations up to 100 μg mL⁻¹. Transepidermal water loss values showed a slight reduction when the formulation …