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Obesity is associated with alterations in the bile acid circulation; and bile acids are essential emulsifiers of cholesterol absorption. Patients with insulin resistance and metabolic syndrome have lower levels of cholesterol absorption when compared to lean healthy weight controls. However, the cholesterol absorption and its correlation with fecal bile acids and fat in human obesity remain unclear. Hence, we aim to study the level of intestinal cholesterol absorption and fecal levels of bile acids and fat in human obesity. We conducted a 4-week nutritional intervention trial of three different metabolic stages for one week over four weeks: 1) Regular diet (RD), 2) high calorie, high fat diet (HCFD)(2700 Kcal/day, 33% from fat) 3) Washout period on RD, and 4) low calorie low fat diet (LCFD)(1200 kcal/day). All the meals were provided by the study. The intestinal cholesterol absorption was measured using serum d7-cholesterol (isotope-labeled cholesterol) levels. On the 5th day of each week, we measured the fasting serum, baseline free and total levels of both d7-cholestero, l and regular (total) cholesterol prior to a meal mixed with 100mg oral dose of d7-cholesterol. 48-hours later, another fasting serum sample was collected to repeat the same measurements. The following equation was used to calculate the change in total, and free cholesterol: total or free delta (D) d7-cholesterol= 48 hours–baseline levels of total or free d7-cholesterol, respectively. A stool sample was collected after 48-hours between day 5 and 7 of every week for fecal bile acids and fecal fat, both measured by HPLC. Statistical analyses included student’s and …