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Thin layer chromatography (TLC) is considered as an efficient analytical technique used for the monitoring and identification of the adsorption behaviour of different amino acids through soil as a stationary phase amended with polyvinyl acetate at different concentration. The study gives complete idea about the mobility of four different uncharged amino acids namely glycine, alanine, tryptophan and glutamine in terms of retention factor by thin layer chromatography. Among four amino acids, highest mobility was found in case of glycine. The order of mobility was found to be in order of glycine > alanine > tryptophan > glutamine in which distilled water has been used as a mobile phase. It has been observed from the results that pH and electrical conductivity also influence mobility of amino acids. The influence of activation temperature and particle size of soil on the mobility of amino acids was also studied.