… of the donor template and Cas9 via covalent linkage increases the correction rates up to
24-fold, … loci, suggesting that covalently linking the donor template to the Cas9 complex provides …

… of CRISPR-Cas9 to … covalent bonds with specific sequences of unmodified single-stranded
DNA (ssDNA) and can function in fusion tags with diverse protein partners, including Cas9 14 …

… our CRISPR-Cas9 photoactivation technology, we constructed a GFP reporter stable cell line
which expresses GFP only when CRISPR-Cas9 … to form a covalent bond between two RNA …

… bonds is generally simple and mild. Additionally, due to the reversible nature of this unique
covalent bond… In this section, we focus on the progress of covalently conjugated Cas9 protein …

… of CRISPR–Cas9 from its … bond showing the cleaved bond highlighted in red. (B) Restriction
endonucleases can hydrolyze the bond via two mechanisms, type I, which involves covalent …

… Here we demonstrate that covalently linking the DNA repair template to Cas9 increases
the ratio of HDR over NHEJ up to 23-fold, and therefore provides advantages for clinical …

… repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) genome editing … Cas9 protein
and guide RNA (gRNA) sequence has recently expanded biomedical application of CRISPR/Cas9 …

… features compared with widely used Cas9, making it an attractive and … , we covalently
conjugated 5′ terminal modified CRISPR RNA (… ) The covalent linkage could prevent the complex …

… During the formation of covalent bonds, the efficiency of RNPs delivery increases due to the
… Therefore, either sgRNA or Cas9 protein can form a covalent bond with the carrier. A bond …

… the action mechanisms of CRISPR/Cas9, its … CRISPR/Cas9-based cancer treatment in
vivo, even the future clinic applications. We first discuss the major characteristics of CRISPR/Cas9 …